Viral RNA Extraction Kit COVID-19
Method 1: Spin Colums
For MANUAL Viral RNA Extraction
Price: $us 199 (For 50 Tests)
We Have The Best Price On The Market!
Method 2: Magnetic Beads Viral RNA Extraction
Compatible With AUTOMATED Viral RNA Extraction Machines
Price: $us 179 (for 50 Tests)
Warranty: 1 YEAR
IMPORTANT: The Difficult RNA Extraction Proccess is NOT needed anymore.
Traditional RNA Extraction:
Viral RNA Extraction Kit (High Purity):
– Fast, Easy and Affortable RNA Extraction Kit.
– Ethanol precipitation is NOT REQUIRED.
– RNA Extraction Kit Ideal for Real-Time RT-PCR, applications COVID-19.
– ARN Extraction Operation Time: 15 to 20 minutes (Columns).
– ARN Extraction Operation Time: 10 to 15 minutes (Magnetic Beads).
– Methods: Manual And Automated
– This kit is compatible with most of the RNA Extraction Automated Equipments
Viral RNA Extraction Kits (Columns) PRICE COMPARISON:
|COMPANY||RXNS||PRICE in $us.|
|Qiagen – 61904||50||$us 286|
|NEB – T2010S||50||$us 314|
|Assay Genie – GN0005||50||$us 789|
|BioCat – 17200-NB||50||$us 470|
|MaxPrecision Lab – V102||50||$us 199|
MaxPrecision Lab has the CHEAPEST PRICE for RNA Extraction Kits on the market.
MaxPrecision Lab has one of the Lowest Prices in the industry. Additionally, we have the capability to do RNA Extraction Kits BULK Production, and in this way we have the ability to beat prices of any other laboratory within the industry.
Viral ARN Extraction Kit Samples (Columns):
All RNA Extraction Kits Samples can be shipped at Room Temperature.
The Number Of Requests For Viral RNA Extraction Kits Is Very High Due to COVID-19 Pandemic.
Therefore, Viral RNA Extraction Kit Samples Get Out Of Stock Frecuently.
Please Make Sure To Take This Into Consideration To Avoid Delays.
Thank you for understanding!
Spin Columns Vs. Magnetic Beads:
|Viral RNA Extraction||Spin Columns||Magnetic Beads|
|Size (Reactions)||50 Rxns**||50 Rxns|
|Price||$us 199||$us 179|
|Format||Manual||Automated and Manual|
|1.5 ml Microcentrifuge Tubes||Required||Required|
|PBS (Phosphate Buffered Saline)||Required||Not required|
|Absolute ethanol (96~100%)||Required||Required|
|Centrifuge Machine||Required||Not required|
|Water bath/dry bath||Not required||Required|
|Magnetic Separator||Not required||Required|
|Extraction Time||20 min||10 min|
|Join The Waiting List||Order Now|
**100 Rxns (determinations available).
*** You can join the waiting list for Spin Columns. Next Production August 31st, 2020.
Magnetic Separation Rack (Sold Separately)
All customers who buy more than 15 kits of MaxBeads Viral RNA Extraction Kits receive 1 Magnetic Separation Rack FOR FREE.
RNA Extraction Kit (Magnetic Beads) Components:
The Viral RNA Extraction Kit by Magnetic Beads can be easily adapted to most of the automated Nucleic Acid Purification Systems.
|Magnetic Bead||2 ml|
|Lysis Buffer||30 ml|
|Wash Buffer||80 ml|
|Release Buffer||20 ml|
➢ Sample: Up to 300 μl of the virus sample
➢ Storage: Room temperature
➢ Absolute EtOH
➢ Magnetic separator
➢ 1.5 ml microcentrifuge tubes
➢ Water bath / Dry bath
Viral RNA Extraction Kit (Magnetic Beads) Protocol:
Step 1 Lysis
1. Transfer up to 300 μl of the virus sample into a 1.5 ml microcentrifuge tube and add 300 μl of the Lysis Buffer.
2. Mix well and incubate at 65°C for 5 minutes. During this time, pre-heat the Release Buffer to 65°C for the Step 4.
3. Add 300 μl of the absolute EtOH to the lysate and mix well.
Step 2 DNA Binding
1. Add 20 μl of the Magnetic Beads. Mix well by gently shaking for 3 minutes.
2.Place the tube in a magnetic separator for 30 seconds.
3. Remove the solution (If the mixture becomes viscous, increase magnetic bead separation time).
Step 3 Wash
1.Add 800 μl of the Wash Buffer and mix well (Following the wash, the mixture will no longer be viscous).
2.Place the tube in a magnetic separator for 30 seconds. Remove the solution.
Step 4 Release
1. Add 200 μl of the Release Buffer (pre-heated to 65°C) and mix well.
2.Incubate for 3 minutes at 65°C (During the incubation, shake the tube vigorously every minute).
3.Place the tube in a magnetic separator for 1 minute.
4. Carefully transfer ONLY the clean portion of the solution to a clean tube.
Are you confused?
Viral ARN Extraction Kit Components(Columns):
|Buffer V1||45 ml|
|Buffer V2 (Add ethanol)||6 ml (45ml)|
|Buffer W1||45 ml|
|Buffer W2 (Add ethanol)||15 ml (60ml)|
|Buffer RE||10 ml|
|Column VN||100 pcs|
|Collection Tubes||100 pcs|
➢ Sample: : Up to 300 µl of the whole blood or Up to 200 μl of virus sample
➢ Storage: Room temperature
➢ Elution volume: 50 μl
➢ 1.5 ml Microcentrifuge tubes(DNase and RNase free)
➢ PBS (Phosphate Buffered Saline, ➢ Absolute ethanol (96~100%)
RNA Extraction Kit (Columns) Protocol:
Step 1 Lysis
1.Transfer up to 200 μl of the virus sample into a 1.5 ml microcentrifuge tube and add 400 μl of the Buffer V1. (If the sample is less than 200 μl, adjust the sample volume to 200 μl with the PBS)
2.Mix well and let it stand at the room temperature for 10 minutes.
Step 2 Nucleic Acid Binding
1.Add 450 µl of the Buffer V2 (ethanol added) to the sample lysate and shake vigorously.
2.Place a Column VN in a 2 ml Collection Tube.
3.Transfer 700 µl of the lysate mixture into the Column VN.
4.Centrifuge at 16,000 x g for 1 minute.
5.Discard the flow-through and place the Column VN back in the same Collection Tube.
6.Transfer the remaining lysate mixture to the Column VN.
7.Centrifuge at 16,000 x g for 1 minute.
8.Discard the flow-through and place the Column VN back in the same Collection Tube.
Step 3 Wash
1.Add 400 µl of the Buffer W1 into the Column VN.
2.Centrifuge at 16,000 x g for 30 seconds.
3.Discard the flow-through and place the Column VN back into the same Collection tube.
4.Add 600 µl of Buffer W2 (ethanol added) into the Column VN.
5.Centrifuge at 16,000 x g for 30 seconds.
6.Discard the flow-through and place the Column VN back into the same Collection tube.
7.Centrifuge at 16,000 x g again for 2 minutes to remove the residual Buffer W2.
Step 4 Elution
1. Place the Column VN in a clean 1.5 ml microcentrifuge tube (DNase and RNase free).
2.Add 50 μl Buffer RE or RNase-free water (pH is between 7.0 and 8.5) to the center
of each Column VN, let it stand for 2 minutes, and centrifuge at 14,000 x g for 2 minutes.