MaximusReady One-Step PCR MasterMix
Catalog Number: MXD01V100RX
Unit Size: 100 rxns (2.5ml)
Also Known As: Premix / Master Mix / Fluorescent Dye
Description
MaximusReady One-Step PCR is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. MaximusReady One-Step PCR is a pre-mixed solution containing Taq DNA polymerase, PCR buffer, dNTP, gel loading dyes, enhancer, and fluorescence dye. MaximusReady One-Step PCR which contains the Taq DNA polymerase is purified from the E. coli. and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. MaximusReady One-Step PCR, which contains the fluorescence dye, is directly detected on LED transilluminator or UV epi-illuminator after the DNA electrophoresis. MaximusReady One-Step PCR mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 50 μl each.
Application
- No post-staining processing of DNA required.
- Direct loading onto the agarose gel for electrophoresis.
- Sensitivity – High degree of sensitivity equivalent to ethidium bromide.
- Speed – Benefit for quick set up of PCR reaction such as colony PCR when screening for positive clones.
- Compatibility – Use blue light or UV to detect the signal.
Size: 100 Reactions ( 2 × 1.25ml )
Storage
Store at 4°C up to 6 months.
Store at RT up to 3 months.
Store at -20°C up to 1 year.
Shipping Temperature: 4°C
Note: MaximusReady One-Step PCR is light sensitive and should be stored and protected from light.
